通过特定部位的素H3甲基转移酶调节染色质结构
S Rea1, F Eisenhaber, D O'Carroll
1Research Institute of Molecular Pathology, The Vienna Biocenter, Austria.
Nature
|August 19, 2000
概括
SUV39H1和Suv39h1是基因组甲基转移酶,它们在氨酸9处修改基因组H3. 这种修改影响了基因调节和线粒分裂,揭示了动态色素调节机制.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 染色体生物学 染色体生物学
背景情况:
- 染色体组织成更高阶结构对于染色体功能和表观遗传基因调节至关重要.
- 高阶染色体被认为是通过基因素尾部的修改启动的,并由非基因素因素建立的.
- 了解这些机制是解读基因表达控制的关键.
研究的目的:
- 为了识别和描述参与高阶染色体形成的基因组甲基转移酶.
- 调查SUV39H1和Suv39h1.1的酶活性和基质特异性.
- 为了阐明素9甲基化 in vivo 的功能后果.
主要方法:
- 在体外生化测试以确定基因组甲基转移酶活性.
- 对催化域的映射,负责组织素甲基化.
- 使用SUV39H1放松调节或破坏Suv39h活性在原生染色质中的体内研究.
主要成果:
- 人类SUV39H1和小鼠Suv39h1被确定为素H3特异性甲基转移酶,向素9.
- 作为催化动机,SET域与相邻的氨酸丰富区域被映射出来.
- 氨酸9甲基化抑制了氨酸10酸化,并受到其他H3尾部修改的影响.
- 在体内,改变的SUV39H1/Suv39h活性会影响H310酸化,并导致异常的线粒分裂.
结论:
- SUV39H1和Suv39h1在素H3氨酸9甲基化中起着至关重要的作用.
- 在H3尾部的特定位点修饰之间存在功能上的相互依赖.
- 这些发现表明调节高阶染色体结构和功能的动态机制.
更多相关视频
10:09Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
Published on: January 26, 2018
8.0K
11:06Chromatin Immunoprecipitation ChIP of Histone Modifications from Saccharomyces cerevisiae
Published on: December 29, 2017
13.4K
相关概念视频
02:25Spreading of Chromatin Modifications
10.0K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
10.0K
02:32Histone Modification
17.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
17.2K
02:32Histone Modification
4.9K
No description available
4.9K
02:41Chromatin Structure Regulates pre-mRNA Processing
8.5K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
8.5K
02:38Heterochromatin
19.0K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
19.0K
02:38Heterochromatin
5.0K
No description available
5.0K