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在受精时,NO对于卵子激活是必要的,也是足够的
R C Kuo1, G T Baxter, S H Thompson
1Neurosciences Program, Stanford University School of Medicine, Stanford University, California 94305, USA.
Nature
|August 19, 2000
概括
精子中的氧化合成酶在受精期间触发卵子激活. 这一发现对于理解受精和改善体外受精等辅助生殖技术至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 生物化学 生物化学
- 细胞信号传输 细胞信号传输
背景情况:
- 对于发育至关重要的卵子激活是受精期间的增加所启动的.
- 引发这种升的精确分子机制和精子衍生因素在很大程度上是未知的.
- 辅助生殖技术,包括体外受精和克隆,强调需要了解早期受精事件.
研究的目的:
- 确定负责启动升和受精时卵子激活的精子衍生因素.
- 研究氧化合成酶及其相关生物活性在受精过程中的作用.
- 为了确定氧化是否是触发卵子激活的关键信号分子.
主要方法:
- 评估了氧化合成酶 (NOS) 在精子后缩体反应中的存在和活性.
- 测量了卵子化水平和受精后的 (Ca2+) 流量.
- 使用了氧化 (NO) 捐赠者和NOS的微注射,以及NO清除剂 (氧血球蛋白),以评估它们对卵子激活的影响.
主要成果:
- 氧化合成酶被发现在高度和活跃在精子在acrosome反应后.
- 在受精后几秒钟观察到卵子化增加,这是在脉冲之前.
- 微注射NO捐赠者或NOS启动了卵子激活,而NO清理阻止了它.
结论:
- 氧化合成酶和与氧化相关的生物活性在适当的时间和地点存在于精子中,作为卵子激活剂.
- 氧化既必要又足以启动卵子激活,并在受精期间增加.
- 这一发现为受精的分子机制提供了关键的见解,并对生殖医学产生了影响.
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