恐惧记忆需要桃体中的蛋白质合成,以便在检索后重新巩固
K Nader1, G E Schafe, J E Le Doux
1W.M. Keck Foundation Laboratory of Neurobiology, Center for Neural Science, New York University, New York 10003, USA. karim@cns.nyu.edu
Nature
|August 30, 2000
概括
重新激活的恐惧记忆变得不稳定,需要新的蛋白质合成来重新巩固. 这种蛋白质合成对于恢复后稳定记忆至关重要,挑战了传统的巩固理论.
科学领域:
- 神经科学是一个神经科学.
- 记忆研究 记忆研究
- 分子生物学分子生物学
背景情况:
- 新形成的记忆是不稳定的,需要蛋白质合成来巩固长期存储.
- 杏仁体 (LBA) 的侧核和基核对于恐惧记忆的形成和存储至关重要.
研究的目的:
- 调查是否巩固的恐惧记忆需要蛋白质合成,以便在重新激活后重新巩固.
- 为了确定重新激活的记忆是否恢复了对蛋白质合成抑制敏感的不稳定状态.
主要方法:
- 在恐惧记忆重新激活后,注入异素 (一种蛋白质合成抑制剂) 进入动物的LBA.
- 在安尼索米辛输注和重新激活后的后期时间点测试记忆保留.
- 与延迟输注或没有重新激活的安尼索米辛效应进行比较.
主要成果:
- 在记忆重新激活后不久注入安尼索米辛会影响后来的记忆回忆,这表明再巩固失败.
- 这种丧失记忆的效应发生不论重新激活发生在调节后1天还是14天.
- 延迟的安尼索米辛输液 (6小时后重新激活) 不会引起记忆丧失,支持对蛋白质合成的时间限制要求.
- 在没有先前的记忆重新激活的情况下,用 anisomycin 给药不会影响记忆保留.
结论:
- 巩固的恐惧记忆在重新激活时进入不稳定状态,需要新的蛋白质合成来重新巩固.
- 这些发现挑战了传统的记忆巩固模型,突出了蛋白质合成依赖于重新激活记忆的再巩固过程.
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