核状蛋白刺激严格控制的细菌促进体:cAMP-CRP与大肠杆菌中的 (p) pGpp regulons之间的联系
J Johansson1, C Balsalobre, S Y Wang
1Department of Microbiology, Umeå University, Sweden.
Cell
|August 31, 2000
概括
该H-NS蛋白质积极调节大肠杆菌中必不可少的基因. spoT基因的突变通过改变严格反应并将其与cAMP-CRP通路联系起来来恢复生长.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌遗传学 细菌遗传学
背景情况:
- H-NS核蛋白对细菌基因调节至关重要.
- 严格的监管控制了基因表达,以应对环境压力.
- 在H-NS,严格的响应和CRP等全球监管机构之间的相互作用尚未完全理解.
研究的目的:
- 研究H-NS在大肠杆菌严格调控基因表达中的作用.
- 确定影响细菌生长速度和严格反应的遗传因素.
- 为了阐明cAMP-CRP模块和严格响应之间的连接.
主要方法:
- 对缺乏H-NS和StpA的大肠杆菌菌株的基因分析.
- 对影响细菌生长的抑制器突变的隔离和表征.
- 基因映射和测序以识别突变 (例如,在 spoT 基因中).
- 对ppGpp合成和稳定的RNA/蛋白质比率的分析.
主要成果:
- H-NS积极调节严格控制的基因.
- 删除H-NS和StpA可以降低细菌生长速度.
- 一种spoT抑制器突变 (spoT(A404E)) 通过减少ppGpp合成来恢复生长.
- 通过严格的反应,crp基因受到负面调节.
- 在抑制菌株中恢复了稳定的RNA/蛋白质比率.
结论:
- H-NS在严格调节的基因的表达中起着积极的作用.
- 在cAMP-CRP模块和大肠杆菌严格反应之间存在直接联系.
- spoT基因是一个关键的调节器,连接细菌生长,严格的反应和全球基因调节.
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