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Replication in Eukaryotes02:31

Replication in Eukaryotes

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Chromosome Structure02:40

Chromosome Structure

A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
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Replication in Eukaryotes01:29

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Updated: Jun 16, 2026

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

酵母染色体复制中的时间顺序

W Burke, W L Fangman

    Cell
    |July 1, 1975
    PubMed
    概括
    此摘要是机器生成的。

    N-甲基-N'-尼特罗-N-尼特罗斯古安丁 (NG) 突变发生在酵母菌中揭示了时间基因组复制. 特定的基因在DNA合成的不同间隔中对NG表现出高峰敏感性,这表明在酵母基因组中有序复制.

    更多相关视频

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
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    Published on: October 21, 2022

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
    07:48

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    相关实验视频

    Last Updated: Jun 16, 2026

    Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
    17:14

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    Published on: December 10, 2012

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
    08:40

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    Published on: October 21, 2022

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
    07:48

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

    Published on: October 11, 2022

    科学领域:

    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.
    • 微生物学 微生物学

    背景情况:

    • 基因对N-甲基-N'-尼特罗-N-尼特罗斯古安丁 (NG) 突变的敏感性与细菌的复制时间相关.
    • 了解基因组复制顺序对于真核细胞生物学至关重要.

    研究的目的:

    • 为了研究单细胞真核生物,Saccharomyces cerevisiae (酵母菌) 中基因组复制的时间顺序.
    • 为了确定特定的基因是否在DNA合成期间在预先确定的时间复制.

    主要方法:

    • 使用N-甲基-N'-尼特罗-N-尼特罗斯瓜尼丁 (NG) 作为一种突变原体来评估基因敏感性.
    • 在指数增长的酵母细胞与抑制DNA合成的细胞中比较突变发生的敏感性.
    • 分析了同步的酵母细胞种群,以确定最大遗传标记器对NG敏感度的不同间隔.

    主要成果:

    • 呈指数增长的酵母细胞比抑制DNA合成的酵母细胞对NG突变更敏感.
    • 同步细胞中的个体遗传标记在DNA合成期间的离散间隔内显示出最大的NG敏感性.
    • 四个染色体中的五个基因的标记物显示出明显的敏感度峰值,表明时间复制的顺序.

    结论:

    • 这些发现表明,酵母基因组中的大多数基因的复制时间顺序.
    • 在酵母染色体DNA的定义区域中,可能存在DNA复制的特定启动点.
    • 这些复制启动点在DNA合成期间的预定时间被激活.