降低细胞质Ca2+) 负荷和改善心脏功能,心脏性冷藏后的几内亚猪隔离心脏
D F Stowe1, S G Varadarajan, J An
1Anesthesiology Research Laboratory, Department of Anesthesiology, Medical College of Wisconsin, and Veterans Affairs Medical Center, Milwaukee, WI, USA.dfstowe@mcw.edu
Circulation
|September 7, 2000
概括
心脏 (CP) 在低温储存期间减少负荷,在重新加热后改善心脏功能. 这项研究将减少的透静与冷藏后更好地恢复心脏压力和效率联系在一起.
科学领域:
- 心血管生理学心血管生理学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 低温提供心脏保护,但可以导致有害的 (Ca2+) 负载和功能损害在重新加热期间.
- 了解细胞溶液Ca2+动态对于优化低温心脏保护策略至关重要.
研究的目的:
- 调查细胞 Ca2+水平变化与冷藏期间和冷藏后心脏功能之间的关联.
- 为了比较心肌 (CP) 与标准克雷布斯-林格 (KR) 溶液对低温存储心脏中的Ca2+处理和功能恢复的影响.
主要方法:
- 在冷却和4小时冷藏3°C期间,用KR溶液或CP溶液 perfused 海豚心脏.
- 监测左心室压力 (LVP),dP/dt,氧气消耗,心脏效率和细胞质Ca2+过渡物 (使用内-1光).
- 在冷藏和随后的再注血之前,期间和之后进行了测量.
主要成果:
- 用单独的KR冷却增加了透和相位Ca2+;CP抑制了相位Ca2+并减弱了透Ca2+的增加.
- 在CP后的再输血导致相位Ca2+ (86%在20分钟) 显著增加,并且与KR相比保留了透静Ca2+ .
- 在再注血过程中,用CP保存的心脏表现出更好的LVP恢复 (126%和50%更好),氧气消耗 (23%更高) 和心脏效率 (38%更高).
结论:
- 心脏症有效地减轻了冷藏期间透缩Ca2+的增加.
- 降低了与CP的Ca2+负载,其特点是储存后较低的透静和较高的收缩Ca2+,在再注血时显著提高了心脏功能.
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