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DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: Jul 13, 2026

Microcontact Printing of Proteins for Cell Biology
09:21

Microcontact Printing of Proteins for Cell Biology

Published on: December 5, 2008

打印蛋白质作为微阵列用于高通量功能的确定.

G MacBeath1, S L Schreiber

  • 1Center for Genomics Research, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA. gavin_macbeath@harvard.edu

Science (New York, N.Y.)
|September 8, 2000
PubMed
概括

研究人员开发了微型蛋白质微阵列,用于高通量查. 这些测试同时快速分析成千上万种蛋白质,使蛋白质相互作用和功能的新发现成为可能.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 高通量蛋白质分析对于理解生物功能至关重要.
  • 目前的方法往往需要大量的样本,而且耗时.
  • 已定义的克隆基因组正在开发用于蛋白质表达和净化.

研究的目的:

  • 开发小型测试,以快速,同时处理数千种蛋白质.
  • 为蛋白质功能的高通量研究创建一个平台.
  • 为了证明蛋白质微阵列在各种生物应用中的实用性.

主要方法:

  • 使用高精度机器人制造互补DNA (cDNA) 微阵列.
  • 在高空间密度的化学衍生玻璃幻灯片上发现了纯化的蛋白质.
  • 确保了蛋白质的共价附着,同时保持了它们的特定相互作用能力.

主要成果:

  • 开发了能够分析极小样本量的蛋白质微阵列.
  • 证明了同时处理数千种蛋白质的能力.
  • 成功选蛋白质-蛋白质相互作用,确定蛋白质激酶基质,并发现小分子标.

结论:

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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

相关实验视频

Last Updated: Jul 13, 2026

Microcontact Printing of Proteins for Cell Biology
09:21

Microcontact Printing of Proteins for Cell Biology

Published on: December 5, 2008

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

  • 蛋白质微阵列为高通量功能蛋白质组学研究提供了强大的工具.
  • 开发的平台有助于快速选和识别分子相互作用.
  • 这项技术在药物发现和生物研究中具有广泛的应用.