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Wet Beveling of Microinjection Needles Utilizing Constant Air Pressure for Feedback on Needle Opening06:00

Wet Beveling of Microinjection Needles Utilizing Constant Air Pressure for Feedback on Needle Opening

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This protocol describes the assembly of a pneumatic system for the delivery of pressurized air to a needle during the process of needle beveling. The protocol further describes the beveling process for creating sharp microinjection needles and how to gauge the relative opening size of the...
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Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels08:12

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This protocol demonstrates in detail how to perform fully endoscopic mitral valve surgery (EMS) with percutaneous cannulation of the groin vessels, using a percutaneous plug-based vascular closure device. Fundamental steps and useful instructions are described in detail for each step.
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This "two-step method" significantly improved the success rate of percutaneous transhepatic choledochoscopy and achieved a better prognosis of intrahepatic and extrahepatic...
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Three-Dimensional Printing Guide Template Assisted Percutaneous Vertebroplasty (PVP)05:39

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Herein, we present a three-dimensional printing guide template for percutaneous vertebraplasty. A patient with a T11 vertebral compression fracture was selected as a case...
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In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses09:31

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Optical coherence tomography (OCT) is a high resolution imaging technique that allows analysis of tissue specific optical properties providing the means for tissue differentiation. We developed needle based OCT, providing real-time imaging combined with on-the-spot tumor differentiation. This publication describes a method for percutaneous, needle based OCT of renal...
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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy11:27

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Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research...
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A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy
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枕头收缩驱动细菌动性的运动.

A J Merz1, M So, M P Sheetz

  • 1Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201-3098, USA.

Nature
|September 19, 2000
PubMed
概括

第四类 pili (Tfp) 强烈收缩,产生显著的力量直接调解细菌细胞运动和表面爬行. 这种回收机制对于细菌的运动性和微殖民地形成至关重要.

科学领域:

  • 微生物学 微生物学
  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.

背景情况:

  • 格拉姆阴性细菌利用抽和社会滑翔运动来进行表面转移.
  • 第四类 pili (Tfp) 对于这些运动机制至关重要,但它们的确切作用尚不清楚.

研究的目的:

  • 阐明IV型 pili (Tfp) 介导细菌运动的机制.
  • 量化Tfp在细菌表面运动过程中产生的力量.

主要方法:

  • 使用激光子来操纵和测量单个细菌细胞及其Tfp所施加的力量.
  • 对Tfp-珠相互作用进行了定量力测量.
  • 在玻璃表面观察到细菌爬行和微殖民地形成.

主要成果:

  • 证明Tfp强力收缩,积极地将细菌细胞拉向微殖民地.
  • 当Tfp与乳珠结合时,测量的收缩力超过80 pN.
  • 证实Tfp介导的运动和收缩发生在大约1微米s(-1) 时,并且取决于蛋白质合成和Pilt蛋白.

结论:

  • 确立了第四类 pili (Tfp) 活跃收缩,产生了相当大的力.

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  • 得出结论,Tfp收缩是驱动细菌运动和表面爬行的直接机制.
  • 突出了Pilt蛋白在Tfp介导的力量产生和运动中的重要性.