在体内检测和测量与翻译相关的蛋白质降解
1Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Science (New York, N.Y.)
|September 23, 2000
概括
新合成的蛋白质可以在折叠之前迅速降解,如果它们具有降解信号. 这种共翻译性蛋白质降解会影响蛋白质成熟和细胞质量控制.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 从核糖体中出现的新生的多可能呈现降解信号.
- 这些信号可能与ubiquitin系统在错误折叠的蛋白质中识别的信号相似.
研究的目的:
- 为了研究活细胞中共翻译性蛋白质降解.
- 用降解信号量化新生蛋白质的降解程度.
主要方法:
- 使用了无处不在的三明治技术.
- 在体内检测并测量了与翻译相关的蛋白质降解.
主要成果:
- 超过50%的具有氨基末端降解信号的新生蛋白质经历了共翻译性降解.
- 退化的蛋白质在达到成熟大小之前被过程性蛋白质分解破坏.
结论:
- 蛋白质折叠与共翻译性降解途径进行动力竞争.
- 新生蛋白质降解是一个重要的细胞过程,影响蛋白质成熟.
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