在S. pombe中,swi1和swi3执行印记,暂停和终止DNA复制
1DHHS, NCI, Division of Basic Sciences Gene Regulation and Chromosome Biology Laboratory, NCI-Frederick Cancer Research and Development Center, Maryland 21702, USA.
Cell
|October 13, 2000
概括
Swi1p和Swi3p蛋白质在交配类型位点 (mat1) 暂停DNA复制分叉,以确保S. pombe的细胞类型切换. 这些因素也有助于在RTS1的复制终止,影响印记.
科学领域:
- * 分子生物学 * 分子生物学
- * 遗传学 遗传学是一门学科
- * 细胞生物学 * 细胞生物学
背景情况:
- 在S. pombe中,细胞类型的切换是一个关键的发育过程.
- * 这种切换依赖于在交配类型位点 (*mat1*) 的特定DNA印记事件.
- *印记是定向的,取决于复制时间和特定的转作用因素.
研究的目的:
- * 阐明了 *swi1p* 和 *swi3p* 在 *mat1* 印记中的作用.
- * 调查swi1p和swi3p在DNA复制终结中的参与.
- * 确定影响复制分叉暂停和终止的因素.
主要方法:
- * 基因查以确定新的终结因素.
- *分析复制叉动态中的 *swi1p* 和 *swi3p* 函数.
- * 检查S. pombe突变体中的印记和终结效率.
主要成果:
- 发现 * * Swi1p * 和 * swi3p * 在 * mat1 * 打印部位中暂停复制叉.
- * 这些因素也在在RTS1终端端结束复制的过程中发挥作用.
- * 一个特定的突变基因基因表现出暂停/打印和终止功能的分离.
结论:
- * *Swi1p* 和 *swi3p* 通过复制叉暂停来促进 *mat1* 印记.
- * 他们参与RTS1终止间接地为印记做出了贡献.
- * 这些发现揭示了新的机制,即swi1p和swi3p调节DNA印记和复制.
相关概念视频
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
02:10Translesion DNA Polymerases
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
03:01The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...