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相关概念视频

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:

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相关实验视频

Updated: May 12, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

核受体核心压缩剂在转录和发育中的组合作用.

K Jepsen1, O Hermanson, T M Onami

  • 1Howard Hughes Medical Institute, Department of Biology, University of California, San Diego, La Jolla 92093, USA.

Cell
|October 13, 2000
PubMed
概括

核受体核抑制剂1 (N-CoR) 对于甲状腺动物的发育至关重要,它既调节了基因抑制,也调节了基因激活. 基因删除研究揭示了N-CoRR.

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Last Updated: May 12, 2026

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Published on: June 21, 2016

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科学领域:

  • 分子生物学分子生物学
  • 发育生物学 发展生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 转录抑制对于元动物的发育至关重要.
  • 像N-CoR和SMRT这样的核心压力器是关键的调节器.
  • 在各种发展过程中,N-CoR发挥着关键作用.

研究的目的:

  • 研究N-CoR在发育过程中的转录调节中的作用.
  • 了解N-CoR在镇压和激活途径中的参与.
  • 阐明核心压缩剂和基因脱乙酶在基因特异性调节中的功能.

主要方法:

  • 在小鼠中进行基因删除研究 (N-CoR基因被删除的小鼠).
  • 分析各种组织和细胞类型的转录模式.
  • 研究N-CoR在核受体介导和REST/NRSF介导镇压中的作用.
  • 检查N-CoR在网红酸反应元件激活中的功能.

主要成果:

  • N-CoR 删除导致中枢神经系统,红细胞和胸细胞系的发育阻断.
  • N-CoR对于核受体和MAD的短期活跃抑制至关重要.
  • 对于由REST/NRSF调解的长期压制事件的一个子集,N-CoR是必需的.
  • 意想不到的是,N-CoR和一个脱乙酶是需要的转录激活的网红酸反应元素.

结论:

  • 在发育过程中,N-CoR是基因特异性转录调节的关键媒介.
  • 核心抑制剂和基因素脱乙酶的特定组合协调基因活性.
  • 这些发现凸显了核心压力在发育过程中的复杂,取决于背景的角色.