相关实验视频
Updated: Jul 16, 2026
10:07
Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
Published on: May 26, 2017
Pax7对于肌性卫星细胞的规范是必需的
P Seale1, L A Sabourin, A Girgis-Gabardo
1Department of Biology, McMaster University, Hamilton, Ontario, Canada.
Cell
|October 13, 2000
概括
配对盒转录因子Pax7对于成年肌肉中的卫星细胞发育至关重要. 它的缺失导致不同的肌肉干细胞种群与改变的造血殖民地形成.
科学领域:
- 肌肉干细胞生物学 肌肉干细胞生物学
- 骨肌肉的再生和再生
- 转录因子函数的功能.
背景情况:
- 卫星细胞是肌肉干细胞,对于肌肉修复至关重要.
- 已知转录因子Pax7参与肌肉发育.
- 在区分卫星细胞与其他肌肉干细胞方面,Pax7的确切作用尚未完全理解.
研究的目的:
- 研究转录因子Pax7在卫星细胞规范中的作用.
- 为了确定Pax7是否能区分卫星细胞和肌肉衍生干细胞.
- 分析Pax7缺失对肌肉干细胞种群的功能后果.
主要方法:
- 代表差异分析以识别Pax7表达式.
- 在现场杂交以检测成人肌肉中的Pax7.
- 细胞培养和电子显微镜以评估卫星细胞的存在.
- 光激活细胞分类 (FACS) 用于量化肌肉干细胞种群.
- 造血殖民地形成试验用于评估干细胞潜力.
主要成果:
- Pax7 特别表达在卫星细胞衍生的神经细胞和成年肌肉卫星细胞中.
- Pax7淘汰赛 (Pax7(-/-)) 鼠完全缺乏骨肌肉中的卫星细胞.
- 肌肉衍生干细胞的比例在Pax7(-/-) 小鼠中保持不变.
- 来自Pax7(-/-) 小鼠的肌肉干细胞显示出显著增加的血液生成殖民地形成能力.
结论:
- 卫星细胞和肌肉衍生的干细胞是不同的细胞群.
- 帕克斯7在从肌肉衍生干细胞中特制卫星细胞中发挥着关键作用.
- Pax7通过限制肌肉干细胞的替代发育途径而起作用,从而促进卫星细胞的同一性.
相关概念视频
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
01:13Formation of Muscle Fibers from Myoblasts
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
01:21Satellite Stem Cells and Muscular Dystrophy
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...