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相关概念视频

Exon Recombination02:32

Exon Recombination

3.1K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.1K

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相关实验视频

Updated: Apr 30, 2026

Recombineering Homologous Recombination Constructs in Drosophila
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Recombineering Homologous Recombination Constructs in Drosophila

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两个重组等离子体的序列组织,其中含有D. melanogaster的主要热冲击诱导蛋白质的基因.

E A Craig, B J McCarthy, S C Wadsworth

    Cell
    |March 1, 1979
    PubMed
    概括
    此摘要是机器生成的。

    研究人员研究了Drosophila的热冲击基因,发现它在基因组中重复了大约七次. 这些基因为70K蛋白编码,并对基因组织和间距进行了详细分析.

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    相关实验视频

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    One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
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    科学领域:

    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.
    • 果虫研究研究 果虫研究

    背景情况:

    • 多虫中主要的热冲击诱导基因对细胞应激反应至关重要.
    • 了解该基因的组织是解读其调节和功能的关键.

    研究的目的:

    • 分析编码Drosophila主要70K热冲击蛋白的基因的组织和结构.
    • 为了确定这些热冲击基因的副本数和基因组位置.

    主要方法:

    • 隔离含有cDNA和染色体DNA序列的复合DNA克隆.
    • DNA:DNA重新关联和现场杂交,以获取副本号码和位置.
    • 基因结构和排列的R循环杂交和限制酶分析.

    主要成果:

    • 70K热冲击基因序列在平分类Drosophila基因组中大约重复7次.
    • 基因序列位于87A和87C位置.
    • 等离子体G3含有三种并列重复的基因 (每种约2100 bp),由约1400 bp的间隔DNA分开;等离子体G5含有该基因的一个副本.

    结论:

    • 在Drosophila中的7万个热冲击蛋白编码基因存在于多个副本中,并被组织成群集.
    • 基因群中的间隔DNA序列显示出变异,表明复杂的调节机制.