两个D. melanogasterDNA片段的物理地图,其中包含编码为70,000达尔顿热冲击蛋白的序列
Cell
|May 1, 1979
概括
研究人员比较了编码热冲击蛋白的Drosophila melanogaster (Dm) 的DNA片段. 他们发现了具有不同序列元素的同类区域,揭示了对基因组织和进化的洞察力.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 德洛索菲拉 (Drosophila melanogaster) 的研究研究
背景情况:
- 两种混合等离子体,56H8和132E3,含有Drosophila melanogaster (Dm) 的DNA序列,与热冲击蛋白mRNA相补充.此前已经被分离出来.
- 了解这些克隆DmDNA段的序列排列对于研究热冲击基因调节至关重要.
研究的目的:
- 为了比较两个克隆的DmDNA段 (56H8和132E3) 中的序列排序.
- 为了阐明编码Drosophila melanogaster中7万达尔顿热冲击蛋白的DNA序列的组织.
主要方法:
- 克隆DNA片段的限制酶分析.
- 交叉杂交实验以评估序列同质性.
- 不同重复分析用于比较DNA结构.
主要成果:
- 这两个克隆的DNA片段来自Dm基因组的非重叠区域.
- 存在同类区域,在56H8中出现一次,在132E3.3中出现两次.
- 每个同源区域包括三个不同的连续序列元素 (x,y,z),形成一个3kB的共同单元.
- 在所有常见单位的2.5kb'z'元素中观察到高序列同质性.
结论:
- 克隆的DNA片段含有同类区域,保留了"z"元素,但具有可变的"x"和"y"元素.
- 这种安排表明Drosophila melanogaster.中热冲击蛋白基因的复杂组织.
- 需要进一步分析以了解这些序列变化的功能影响.
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