对人体内皮细胞的C反应性蛋白直接有助于炎症作用
V Pasceri1, J T Willerson, E T Yeh
1Department of Internal Medicine, University of Texas Health Science Center, Houston, TX, USA.
Circulation
|November 1, 2000
概括
C-反应蛋白 (CRP) 显著增加了人体内皮细胞中的粘附分子表达,这表明它在动脉样硬化炎症和潜在的治疗向中发挥了作用.
科学领域:
- 心血管生物学 心血管生物学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- C-反应蛋白 (CRP) 是一种关键的急性相反应剂,也是冠状动脉心脏病的重要危险因素.
- CRP对血管细胞功能,特别是内皮细胞的特定影响仍然在很大程度上未被描述.
研究的目的:
- 研究CRP对人类内皮细胞粘附分子表达的影响.
- 确定CRP是否有助于与动脉样硬化有关的炎症过程.
主要方法:
- 人的静脉和冠状动脉内皮细胞与重组的人类CRP进行了化.
- 血管细胞粘附分子 (VCAM-1),细胞间粘附分子 (ICAM-1) 和E-selectin的表达用流动细胞计量来量化.
- 用人血清和没有人血清进行了实验,以评估CRP对血清因子的依赖性.
主要成果:
- CRP (10微克/毫升) 在内皮细胞中显著上调ICAM-1,VCAM-1和E-选择素的表达,其效果与互白素-1β.
- CRP诱导的粘附分子表达取决于培养基中存在的人类血清.
- 在冠状动脉内皮细胞中观察到剂量依赖的效应,最大诱导在50微克/毫升CRP.
结论:
- 在人体内皮细胞中,CRP直接诱导粘附分子的表达,这取决于血清的存在.
- 这些发现支持CRP有助于动脉样硬化的炎症成分的假设.
- 向CRP可能为治疗动脉样硬化提供一种新的治疗策略.
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