在小核盒 C/D RNP 和 spliceosomal U4 snRNP 之间共享一个共同的核心 RNP 结构
N J Watkins1, V Ségault, B Charpentier
1Max-Planck-Institut für Biophysikalische Chemie, Abteilung Zelluläre Biochemie, Göttingen, Germany.
Cell
|November 18, 2000
概括
Snu13p蛋白与盒子C/D小核核RNAs (snoRNAs) 结合,这些RNAs对于RNA处理至关重要. 削减Snu13p会破坏RNA代谢,这表明它在核糖蛋白复合体中起着保留的作用.
科学领域:
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
- 遗传学 是一个遗传学.
背景情况:
- 框 C/D 小核核RNAs (snoRNAs) 对于指导2'-O-甲基化和处理核糖体RNA至关重要.
- 众所周知,Snu13p是U4/U6.U5三snRNP的蛋白成分,它参与了拼接.
- 在RNA代谢中Snu13p的结构和功能作用尚未完全理解.
研究的目的:
- 调查Snu13p与盒子C/DsnoRNAs的相关性.
- 确定Snu13p-snoRNA相互作用在RNA代谢中的功能意义.
- 探索U4 snRNP和盒子C/D snoRNP之间的进化关系.
主要方法:
- 酵母中Snu13p的遗传耗尽. 在酵母中Snu13p的遗传耗尽.
- 在体外结合测试以评估Snu13p-box C/D动机相互作用.
- 盒子C/D基因和U4 snRNA结合位点的结构分析.
主要成果:
- 发现Snu13p与盒子C/D的snoRNAs有关.
- 酵母中Snu13p的遗传枯竭导致RNA代谢的显著缺陷.
- 盒子C/D图案与U4 snRNA中的Snu13p结合部位具有结构上的相似性,并且在体外结合Snu13p.
结论:
- Snu13p在盒子C/DsnoRNP的功能中起着至关重要的作用,影响整体RNA代谢.
- U4 snRNP与盒子C/D snoRNP之间的结构和功能并行表明它们具有共同的进化起源.
- 对于snRNP和snoRNP来说,Snu13p充当了一种保存的陪伴蛋白.
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The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals.
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...
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