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开发一种通过冠状动脉进行细胞移植的新方法
K Suzuki1, N J Brand, R T Smolenski
1Department of Cardiothoracic Surgery, National Heart and Lung Institute, Imperial College School of Medicine at the Heart Science Centre, Harefield Hospital, Middlesex, UK. Ken.Suzuki@harefield.nthames.nhs.uk
Circulation
|November 18, 2000
概括
冠心内输液为心力衰竭提供了一种有效的细胞移植方法,通过使心肌内全球细胞分布成为可能,超过了传统的注射. 这种技术在心脏手术中显示出临床应用的潜力.
科学领域:
- 再生医学是一种再生医学.
- 心血管生物学 心血管生物学
- 细胞疗法细胞疗法
背景情况:
- 细胞移植是对末期心力衰竭的有希望的治疗方法.
- 直接肌肉内注射限制了心肌中的全球细胞分布.
- 冠内输液为心脏细胞输送提供了一种新的方法.
研究的目的:
- 开发和评估一种用于心脏细胞移植的新型冠状动脉输液方法.
- 评估移植细胞在心脏组织中的效率和整合.
主要方法:
- 产生了表达ss-galactosidase (ss-gal) 的L6大鼠骨肌细胞.
- 通过冠状动脉注入 10^6 ss-gal-表达细胞到经过扩张的老鼠心脏中.
- 进行异型心脏移植并分析细胞移植和分化.
主要成果:
- 在第3天,每个心脏大约有5x10^5个移植细胞,到28日增加到5x10^6个.
- 在心脏层中分布的ss-gal-阳性细胞在左侧和右侧冠状动脉区域.
- 移植的细胞分化成肌管,与本地心肌结合,并表达Connexin 43;没有观察到心脏功能下降.
结论:
- 为心脏细胞移植开发了一种高效的冠心内输液方法.
- 这种技术促进了对心脏的全球细胞供应.
- 在心脏手术期间在临床环境中可能适用.
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