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相关概念视频

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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相关实验视频

Updated: Jul 15, 2026

Immunofluorescence Microscopy of &#947;H2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
10:47

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对通过NBS1和玛-H2AXX进行RAG介导的VDJ切割的反应.

H T Chen1, A Bhandoola, M J Difilippantonio

  • 1Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Science (New York, N.Y.)
|December 9, 2000
PubMed
概括

在T细胞受体基因发育过程中,尼米根断裂综合征蛋白 (NBS1) 和基因素玛-H2AX监测DNA断裂. 这种监测可以预防转位和淋巴细胞癌症.

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Immunofluorescence Microscopy of &#947;H2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 免疫学 免疫学 免疫学

背景情况:

  • 影响DNA损伤反应的遗传性疾病在抗原受体位点表现出高的转位率.
  • 这些疾病与对淋巴细胞恶性瘤的敏感性增加有关.

研究的目的:

  • 调查尼日梅根断裂综合征蛋白 (NBS1) 和素玛-H2AX在VDJ重组诱导的DNA双链断裂 (DSB) 中的作用.
  • 为了确定NBS1和gamma-H2AX是否参与预防T细胞发育期间的瘤转位.

主要方法:

  • 在DNA双链断裂 (DSB) 部位对NBS1和玛-H2AX的定位研究.
  • 在发育中的胸细胞中分析核焦点的形成和与T细胞受体α位点的同位.
  • 研究重组激活基因 (RAG) 蛋白质介导的VDJ裂变.

主要成果:

  • NBS1和玛-H2AX与辐射诱导的DSB和VDJ重组诱导的DSB有关.
  • 在发育中的胸细胞中,NBS1和gamma-H2AX形成核焦点,与T细胞受体α位合.
  • 这种局部化发生在响应RAG蛋白介导的VDJ裂变时.

结论:

  • NBS1和gamma-H2AX在识别VDJ重组过程中产生的DNA断裂方面发挥着作用.
  • 通过NBS1和gamma-H2AX对T细胞受体重组中间体的监测至关重要.
  • 这种机制对于预防瘤转位和随后的淋巴细胞恶性瘤可能很重要.