不匹配修复基因频繁水平转移的进化影响
E Denamur1, G Lecointre, P Darlu
1INSERM U 458 Hôpital Robert Debré 48 boulevard Sérurier 75935 Cedex 19, Paris, France.
Cell
|December 15, 2000
概括
在自然种群中的细菌不匹配修复 (MMR) 缺陷留下了明显的基因组印记. MMR基因表现出马赛克主义,这表明水平基因转移通过改变突变和重组率来帮助适应性进化.
科学领域:
- 微生物遗传学微生物遗传学
- 进化生物学是进化的生物学.
- 基因组学就是基因组学.
背景情况:
- 遗传多样性源于突变和重组,由自然选择塑造.
- 缺乏细菌不匹配修复 (MMR) 的突变体表现出高的突变和重组率,并且在自然环境中很常见.
- 这项研究调查了细菌中自然发生的MMR缺乏症的基因组特征.
研究的目的:
- 为了确定MMR缺乏是否在细菌基因组序列上留下可检测的印记.
- 探索埃舍里奇亚大肠杆菌中MMR基因马赛克的进化影响.
主要方法:
- 来自天然大肠杆菌分离物中的MMR基因的比较分子系谱.
- 与家政基因相比,MMR基因中的序列马赛克的分析.
主要成果:
- MMR基因表现出显著的序列马赛克,表明其起源来自不同的基因谱系.
- 这种马赛克主义比家政基因更明显.
- MMR 基因的水平基因转移与MMR缺陷菌株中观察到的超重组表型相关.
结论:
- MMR基因的序列马赛克是适应性进化的潜在标志.
- 重复失去和重新获得MMR基因功能可能会调节突变和重组率.
- 这种机制有助于细菌在自然环境中的适应.
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