c-Jun和JunB对抗性地控制皮肤中细胞因子调节的介质细胞-表皮相互作用
A Szabowski1, N Maas-Szabowski, S Andrecht
1Division of Signal Transduction and Growth Control Deutsches Krebsforschungszentrum Im Neuenheimer Feld 280 69120, Heidelberg, Germany.
Cell
|December 15, 2000
概括
研究人员发现,纤维细胞中的AP-1亚单元对抗性地控制皮肤细胞的生长和分化. 这种由IL-1调节的相互作用会影响表皮再生和组织平衡.
科学领域:
- 发育生物学 发展生物学
- 细胞信号传输 细胞信号传输
- 皮肤病学 皮肤病学
背景情况:
- 介质细胞和上皮细胞的相互作用对于器官发育和组织维护至关重要.
- 这种细胞交叉交谈依赖于细胞表面蛋白质和可溶性因子,通常由基因转录控制.
- 转录因子AP-1家族在这些细胞过程中发挥着作用.
研究的目的:
- 在皮肤生物学中研究AP-1转录因子二分体的特定功能.
- 了解由纤维细胞介导的对膜信号机制,控制状细胞的行为.
主要方法:
- 使用原始人类角质细胞和转基因小鼠纤维细胞 (野生型,c-jun(-/-),junB(-/-) 重建皮肤组织.
- 纤维细胞介导的对膜信号的分析,影响状细胞的增殖和分化.
- 研究介素-1 (IL-1) 在调节关键生长因子中的作用.
主要成果:
- 在纤维细胞中确定了c-Jun和JunB AP-1亚单元之间的对抗功能.
- 证明这种对抗性会影响纤维细胞介导的角质细胞增殖和分化的控制.
- 追溯该机制到依赖IL-1的皮细胞生长因子 (KGF) 和粒细胞巨细胞殖民地刺激因子 (GM-CSF) 的调节.
结论:
- 纤维细胞中AP-1亚单元的相对激活指导了表皮再生.
- 通过AP-1子单元进行非细胞自主调节对于维持皮肤组织平衡至关重要.
- 特定的AP-1二元活性会影响控制上皮细胞行为的副因子的产生.
相关概念视频
MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Cell-matrix's Response to Mechanical Forces
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
Renewal of Skin Epidermal Stem Cells
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
Role of Skin in Vitamin D Synthesis
The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin D3(cholecalciferol).
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin D3(cholecalciferol).


