阿拉比多普西斯的转录因子:在真核生物中进行全基因组的比较分析
J L Riechmann1, J Heard, G Martin
1Mendel Biotechnology, 21375 Cabot Boulevard, Hayward, CA 94545, USA. jriechmann@mendelbio.com
Science (New York, N.Y.)
|December 16, 2000
概括
阿拉比多普西斯 (Arabidopsis thaliana) 拥有超过1500个转录因子,有许多植物特异性家族. 对比分析揭示了真核细胞中转录调节的进化分歧,突出了谱系特定的DNA结合域组合.
科学领域:
- 进行比较的基因组学.
- 分子进化的分子进化.
- 转录因子家族 转录因子家族
背景情况:
- 阿拉比多普西斯莉亚的基因组测序为大规模比较分析提供了基础.
- 转录因子 (TF) 是基因表达的关键调节者,控制基本的生物过程.
- 了解TF进化提供了对生命多样化的见解.
研究的目的:
- 对Arabidopsis thaliana中的转录调节剂进行比较分析,与其他真核生物王国中的调节剂进行比较分析.
- 研究转录因子家族的进化起源和多样化.
- 探索DNA结合域及其安排的保护和分歧.
主要方法:
- 在Arabidopsis thaliana中对转录因子的全基因组识别和分类.
- 对Arabidopsis TF家族与其他真核细胞系中的正统家族进行比较分析.
- 对保存的DNA结合域及其组合模式的生物信息分析.
主要成果:
- 阿拉比多普西斯塔利亚纳编码超过1500个转录因子,其中约45%属于植物特异性家族.
- 所有真核生物共同的TFs在保存的DNA结合域之外显示有限的相似性.
- 观察到保存的DNA结合域具有显著的谱系特异性安排.
结论:
- 阿拉比多普西斯·塔利亚纳基因组包含大量的转录因子,包括许多植物特异性因子.
- 进化过程在转录调节中产生了多样性,特别是通过对保存域的组合性使用.
- 比较基因组学揭示了在真核生物王国的转录因子家族中不同的进化轨迹.
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