相关实验视频
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Analyzing and Building Nucleic Acid Structures with 3DNA
Published on: April 26, 2013
基因组范围内的DNA结合蛋白的位置和功能
1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.
概括
研究人员开发了一种新的微阵列方法,在体内绘制DNA结合蛋白的地图. 这项技术识别了由特定激活器控制的基因,揭示了酵母中的新功能和调节网络.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 系统生物学 系统生物学
背景情况:
- 了解DNA结合蛋白的功能对于破译基因表达和基因组维护至关重要.
- 确定这些蛋白质的体内染色体位置对于这种理解至关重要.
研究的目的:
- 开发和应用一种新的微阵列方法,用于全基因组的DNA结合蛋白质定位.
- 研究酵母中的转录激活剂Gal4和Ste12的直接点和调控作用.
主要方法:
- 开发一种基于微阵列的方法,用于全基因组DNA结合蛋白的定位分析.
- 位置数据与基因表达特征的整合.
- 在酵母中监测转录激活剂Gal4和Ste12的结合.
主要成果:
- 对Gal4和Ste12的全基因组结合位点的识别.
- 蛋白质结合与基因表达变化的相关性,以响应环境线索 (碳源,交配费洛蒙).
- 发现了Gal4和Ste12的协调调节路径和以前未知的功能.
结论:
- 全基因组位置分析是研究基因调节网络的强大工具.
- 这种方法促进了对基因功能和基因组维护机制的研究.
- 这项研究揭示了关于特定转录激活剂在细胞反应中的作用的新见解.
相关概念视频
Organization of Genes
Overview
Nucleic Acids
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
Organization of Genes
Overview
DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
Structure of a Gene
A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...

