相关实验视频

Updated: May 6, 2026

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

Published on: August 8, 2014

瘤坏死因子-α (TNF-α) 在小鼠急性病毒性心肌炎中起着保护作用:一项使用缺乏TNF-α的小鼠进行的研究

H Wada¹, K Saito, T Kanda

¹Department of Laboratory Medicine, Gifu University School of Medicine, Gifu, Japan. wadah@cc.gifu-u.ac.jp

|February 7, 2001

概括

瘤亡因子-α (TNF-α) 对于对抗病毒性心肌炎至关重要. 它的缺席严重降低了生存率,而它的管理改善了结果,突出了它在急性病毒性心脏感染中的保护作用.

科学领域:

心血管研究研究心血管研究
免疫学免疫学免疫学
病毒学病毒学

背景情况:

心脏中的瘤缩因子-α (TNF-α) 表达与病毒性心肌炎有关.
在病毒性心肌炎中TNF-alpha的确切病理生理作用尚不清楚.

研究的目的:

阐明TNF-α在病毒性心肌炎进展中的作用.
研究TNF-α对病毒性心脏感染的保护机制.

主要方法:

使用TNF-alpha基因缺陷 (TNF-alpha(-/-)) 的小鼠和野生类型 (TNF-alpha(+/+)) 的对照.
使用脑肌心炎病毒 (EMCV) 感染诱导的急性心肌炎.
用于重组人类TNF-alpha来评估存活率和病毒载量.

主要成果:

与TNF-alpha(+/+) 小鼠 (67%) 感染EMCV后的小鼠相比,TNF-alpha(-/-) 小鼠的生存率显著较低 (0%).
重组TNF-alpha的剂量依赖性改善了TNF-alpha在小鼠中的生存率.
较高的病毒标位和减少的心肌炎症变化在TNF-alpha{-/-}小鼠中被观察到,并减少了粘附分子表达.

结论:

TNF-α对于粘附分子表达和白细胞招募到炎症部位至关重要.
缺少TNF-alpha会影响病毒性心肌炎中传染病原体的消除.
在病毒性心肌炎的急性阶段,TNF-alpha起着关键的保护作用.

更多相关视频

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody mAb by Neutralizing TNF Using an In Vitro Bioanalytical Method

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody mAb by Neutralizing TNF Using an In Vitro Bioanalytical Method

Published on: September 16, 2017

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

相关概念视频

Myocarditis I: Introduction

Myocarditis I: Introduction

Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...

Myocarditis II: Clinical Features and Diagnostic Tests

Myocarditis II: Clinical Features and Diagnostic Tests

Myocarditis is an inflammation of the heart muscle. The symptoms vary widely, encompassing asymptomatic presentations to severe, acute manifestations.Clinical PresentationAsymptomatic cases: In some instances, myocarditis may be asymptomatic, with the infection resolving without intervention. These cases often go undetected unless discovered incidentally through diagnostic imaging or tests conducted for other reasons.General Early Symptoms: Early symptoms of myocarditis are non-specific and can...

Myocarditis III: Medical Management

Myocarditis III: Medical Management

Myocarditis: Comprehensive Medical ManagementMyocarditis, the heart muscle inflammation, requires a comprehensive medical management strategy that addresses the underlying cause, provides supportive care, manages symptoms, and reduces cardiac workload.Infections and Autoimmune CausesAdminister appropriate antimicrobial therapy when an infectious agent causes myocarditis. For instance, penicillin treats infections caused by Group A Streptococcus. In cases where autoimmune processes are...