通过Slit及其机器人受体进行短距离和远距离引导:机器人受体的组合代码控制横向位置
J H Simpson1, K S Bland, R D Fetter
1Howard Hughes Medical Institute, Division of Neurobiology, Department of Molecular and Cell Biology, 519 LSA, University of California, Berkeley CA 94720, USA.
Cell
|February 13, 2001
概括
在Drosophila中的Robo受体的组合代码通过响应Slit梯度来控制轴突指导. 这个系统精确地绘制了纵向路径,使用远程和短程线索来准确地绘制地形.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 裂蛋白作为驱逐剂,指导Drosophila中枢神经系统 (CNS) 中的轴突.
- 裂纹在中线起到短距离驱虫作用,在侧面区域起到长距离化学驱虫作用.
- 轴突导航到特定的横向位置的精确机制仍然不完全理解.
研究的目的:
- 为了研究机器人受体在中介远程裂纹指导中的作用.
- 阐明机器人受体的组合代码如何确定中枢神经系统中轴突的横向位置.
- 了解远程和短程线索之间的相互作用,以建立精确的轴突地形.
主要方法:
- 分析不同中侧位置的轴突中Robo受体 (Robo,Robo2,Robo3) 的基因表达模式.
- 基因操纵,包括基因去除 (Knockout) 和外宫表达,机器人2和机器人3.
- 观测和分析轴突路径的发现,以应对这些基因操纵,使用Drosophila作为模型生物.
主要成果:
- 不同侧面位置的轴突表达了Robo受体的不同组合:中间轴突表达了Robo,中间轴突表达了Robo和Robo3,侧面轴突表达了Robo,Robo2和Robo3.
- 机器人2或机器人3功能丧失导致横向轴突向中枢神经中枢神经中枢神经错误导航.
- 在中间轴突上Robo2或Robo3的异位表达诱导了它们不适当的横向投射.
结论:
- 机器人受体的组合代码解释了Slit梯度,以决定Drosophila中枢神经系统中轴突投射的宽侧区域.
- 这种机器人受体代码与局部线索相结合,确保了纵向轴突通路的精确地形组织.
- 这些发现揭示了建立神经电路架构所必需的复杂指导机制.
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