细胞素介导转位 (CMT):一种功能相当于格拉姆阳性细菌中的III型分泌物
J C Madden1, N Ruiz, M Caparon
1Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Cell
|February 13, 2001
概括
像Streptococcus pyogenes这样的阳性细菌使用一种涉及Streptolysin O (SLO) 的新途径来注射效应蛋白. 这种细胞酶介导转位 (CMT) 机制是病原体毒性的关键.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- 第三类分泌系统对于阳性细菌的毒性至关重要,但它们的机制仍然不太清楚.
- 斯氏菌 (Streptococcus pyogenes) 是一个重要的格拉姆阳性病原体,对各种人类疾病负责.
研究的目的:
- 为了阐明由格拉姆阳性病原体Streptococcus pyogenes注射效应蛋白的机制.
- 为了研究Streptolysin O (SLO) 在调解蛋白质转移到宿主细胞中的作用.
- 为了建立一种新的格式,用于阳性细菌分泌.
主要方法:
- 研究了SLO和效应蛋白之间的相互作用.
- 利用基于细胞的测试来证明蛋白质转位.
- 分析了SLO和效应因子SPN的协同细胞毒性.
主要成果:
- 描述了一种新的注射途径,用于使用SLO,一种依赖于胆固醇的细胞解酶的阳性细菌.
- 证明了效应因子SPN (S. pyogenes NAD-glycohydrolase) 通过极化过程通过SLO孔位转移.
- 表明SLO和SPN在诱导细胞毒性方面具有协同作用,SPN产生循环ADP-ribose.
结论:
- 细胞质素介导转位 (CMT) 是一种新的机制,用于Gram阳性病原体的效应物传递.
- 通过SLO介导的转位提供了一个功能相当于Gram阳性细菌的III型分泌.
- 这一发现为对胆固醇依赖性细胞酶的功能及其在细菌病原发生中的作用提供了新的见解.
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