流动 (剪切应力) 诱导的内皮依赖扩张在缺乏编码双素的基因的小鼠中发生变化
L Loufrani1, K Matrougui, D Gorny
1Institut National de la Santé et de la Recherche Médicale (INSERM) U 541, IFR6, Université Paris VII, France.
Circulation
|February 15, 2001
概括
缺乏双素的小鼠表现出正常的血管反应,除了流动诱导的扩张减少了50-60%. 这表明dystrophin对于动脉中剪切压力机械传导至关重要.
科学领域:
- 心血管生理学心血管生理学
- 肌肉生物学 肌肉生物学
- 机械生物学 机械生物学
背景情况:
- 消极蛋白对于条纹肌肉中的机械转导至关重要.
- 它在血管功能中的作用,特别是对压力和流量的反应,仍然未被探索.
- 这项研究研究了缺失双素对动脉内皮细胞和光滑肌肉细胞的影响.
研究的目的:
- 为了研究双素在血管对压力和流量的反应中的作用.
- 为了检查缺乏双素的小鼠 (mdx小鼠) 的动脉中的内皮和肌肉反应.
主要方法:
- 从mdx和野生类型小鼠中分离的动脉和中腔阻力动脉.
- 使用动脉造影仪来控制内压力和流量.
- 评估压力和流量诱导的血管度和扩张反应.
主要成果:
- 缺少双素并没有影响血压或压力诱导的音调.
- 在mdx小鼠的动脉中,流量 (剪切应力) 诱导的扩张显著减少 (50-60%).
- 双素的缺失损害了NG-nitro-L-arginine甲基敏感的流量扩张,表明剪切应力信号传导中的缺陷.
结论:
- 双氨酸在动脉内皮细胞内的剪切压力机械传导中发挥着特定的作用.
- 在缺乏双氨酸的小鼠中,血管对流量的反应受损可能会加剧诸如杜申尼肌肉发育不良等疾病的器官损伤.
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