通过自身骨髓移植增加产后新血管化
S Shintani1, T Murohara, H Ikeda
1Cardiovascular Research Institute and the Department of Internal Medicine III, Kurume University School of Medicine, Kurume, Japan.
Circulation
|February 15, 2001
概括
将自身骨髓单核细胞 (BM-MNCs) 移植到缺血组织中,可以促进新的血管形成. 这一策略增强了血液流动和毛细血管密度,为治疗性新血管化提供了有前途的方法.
科学领域:
- 再生医学是一种再生医学.
- 血管生物学 血管生物学
- 细胞疗法细胞疗法
背景情况:
- 内皮原生细胞 (EPC) 在成人外周血液中发现,被认为来自骨髓 (BM).
- 这项研究调查了BM单核细胞 (BM-MNCs) 是否能够产生功能性的EPC.
- 该研究还探讨了自性BM-MNC移植的潜力,以改善缺血后肢疾病中的血管生成.
研究的目的:
- 确定骨髓单核细胞 (BM-MNCs) 是否可以分化为功能性内皮细胞 (EPCs).
- 在子后肢缺血模型中评估自主BM-MNC移植在促进血管生成和附带血管形成方面的疗效.
主要方法:
- 子BM-MNCs被分离和培养,通过乙化LDL吸收,氧化 (NO) 释放和特定标记表达来评估EPC差异化.
- 尾肢缺血是在子中通过手术诱导的.
- 用光标记的自主BM-MNCs或BM-fibroblasts被移植到缺血组织中,随后使用显微镜和血管学对细胞结合和新血管化的分析.
主要成果:
- 在实验室中,BM-MNCs成功生成了表现出特征标记和功能的EPC.
- 移植的BM-MNCs集成到缺血后肢组织的毛细血管网络中.
- 与对照组和BM纤维细胞组相比,接受BM-MNC移植的子显著增加了附带血管形成,毛细血管密度和血液 perfusion.
结论:
- 自主性BM-MNC可以分化为功能性的EPC.
- 自主性BM-MNC的直接移植是治疗性新血管化缺血组织的有效策略.
- 这种方法支持"治疗性血管生成"的概念,用于治疗缺血性疾病.
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