在6.5A分辨率下,ClC型化物通道的投影结构6.5A分辨率
J A Mindell1, M Maduke, C Miller
1Department of Biochemistry, Howard Hughes Medical Institute, Basic Medical Science Research Center, Brandeis University, Waltham, Massachusetts 02454, USA. jmindell@brandeis.edu
Nature
|February 24, 2001
概括
研究人员使用冷电子显微镜可视化了一个 prokaryotic ClC 化物通道. 这种结构分析揭示了二维通道复合体内充满水的毛孔,为离子运输机制提供了洞察力.
科学领域:
- 生物物理学的生物物理.
- 结构生物学 结构生物学
- 分子生物学分子生物学
背景情况:
- 化通道 (ClC) 对于真核生物中多样化的细胞功能至关重要.
- 这些通道调节电刺激,血压和细胞酸化.
- 以前已经表达和净化了一种 prokaryotic ClC 通道同类物.
研究的目的:
- 在高分辨率下确定ClC通道蛋白的结构.
- 通过ClC通道调查离子导电的结构基础.
主要方法:
- 将ClC通道蛋白重构成脂双层膜.
- 形成二维蛋白质晶体的过程.
- 低温电子显微镜 (cryo-EM) 分析.
主要成果:
- 成功形成了ClC通道的二维晶体.
- 获得了ClC通道综合体的6.5 Å分辨率投影结构.
- 在二维通道结构中识别了轴外的充满水的毛孔.
结论:
- 结构数据为理解ClC通道功能提供了分子基础.
- 这项工作推动了对离子通道机制及其在生物系统中的作用的研究.
相关概念视频
Types of Non-structural Cracks in Concrete
507
Non-structural cracks are primarily of three types: plastic, early-age thermal, and drying shrinkage cracks. Plastic cracks are further classified into plastic shrinkage cracks and plastic settlement cracks.
Plastic shrinkage cracks typically form within hours after the concrete is poured. The concrete's surface dries faster than the bottom, creating tensile stress that the still-plastic concrete cannot withstand, leading to diagonal or randomly patterned cracks on the concrete surface.
Plastic shrinkage cracks typically form within hours after the concrete is poured. The concrete's surface dries faster than the bottom, creating tensile stress that the still-plastic concrete cannot withstand, leading to diagonal or randomly patterned cracks on the concrete surface.
507
Fischer Projections
16.6K
Learning to draw Fischer projections of molecules and understanding their relevance plays a crucial role in the visual depiction of organic molecules. A Fischer projection is a two-dimensional projection on a planar surface to simplify the three-dimensional wedge–dash representation of molecules. This is especially helpful in the case of molecules with multiple chiral centers that can be difficult to draw. Here, all the bonds of interest are represented as horizontal or vertical lines. While...
16.6K
Ion Channels
91.5K
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
91.5K
Newman Projections
21.1K
Different notations are used to represent the three-dimensional structure of molecules on two-dimensional surfaces. One of the most commonly used representations is the dash-wedge formula. The dashed wedges, solid wedges, and the plane lines indicate the groups situated behind the plane, coming out of the plane, and in the plane, respectively.
The organic molecules rotate across the single bonds leading to numerous temporary three-dimensional structures of varying energy known as...
The organic molecules rotate across the single bonds leading to numerous temporary three-dimensional structures of varying energy known as...
21.1K
Accessory Structures of the Skin: Hair Growth and Types
2.4K
Hair growth begins with the production of keratinocytes by the basal cells of the hair bulb. As new cells are deposited at the hair bulb, the hair shaft is pushed through the follicle toward the surface. Keratinization is completed as the cells are pushed to the skin surface to form the shaft of hair that is externally visible. The external hair is completely dead and composed entirely of keratin. Hair can be cut or shaven without damaging the hair structure because the cut is superficial. Most...
2.4K
Structural Isomerism
21.7K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
21.7K


