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相关概念视频

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
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Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

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相关实验视频

Updated: May 11, 2026

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
08:53

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Published on: February 18, 2011

一个含有结构性DNA结合成分的人类BRCA2复合体影响细胞周期进展.

L Y Marmorstein1, A V Kinev, G K Chan

  • 1The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA.

Cell
|February 24, 2001
PubMed
概括
此摘要是机器生成的。

研究人员在BRCA2复合体中确定了一个BRCA2-关联因子35 (BRAF35). 这种BRAF35蛋白与凝聚色素结合,并可能调节乳腺癌易感性细胞循环进展.

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Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

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Published on: April 28, 2021

相关实验视频

Last Updated: May 11, 2026

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
08:53

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Published on: February 18, 2011

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
09:24

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 细胞生物学 细胞生物学

背景情况:

  • 人类BRCA2基因的生殖基因突变与乳腺癌风险增加有关.
  • 目前尚不完全了解BRCA2蛋白的确切功能.
  • 缺少BRCA2的小鼠细胞表现出染色体异常.

研究的目的:

  • 为了研究BRCA2蛋白的功能.
  • 为了识别BRCA2复合体的组成部分.
  • 探索BRCA2在细胞过程中的作用.

主要方法:

  • 大型含BRCA2复合体的分离和特征.
  • 一个DNA结合元件的识别和分析,BRAF35.
  • 在小鼠胚胎中对BRAF35mRNA的表达分析.
  • 免疫光染色以评估BRAF35/BRCA2局部化情况.
  • 抗体微注射以研究功能性作用.

主要成果:

  • 一个2 MDa的BRCA2复合体被分离出来,其中包含一种名为BRAF35.5的DNA结合蛋白.
  • BRAF35 具有用于 DNA 结合的 HMG 域和一种类似素的线圈-线圈域.
  • BRAF35的mRNA表达反映了BRCA2的表达,在繁殖的胚胎组织中最高.
  • 在线分裂过程中,BRAF35/BRCA2复合体与凝结色素局部化.
  • 抗体微注射表明该复杂物影响细胞循环的进展.

结论:

  • BRAF35是BRCA2复合体的一个新型DNA结合成分.
  • BRCA2/BRAF35复合体与染色质相关,可能在细胞循环调节中发挥作用.
  • 对BRCA2/BRAF35复合体的进一步研究可以阐明乳腺癌易感性背后的机制.