来自小浮游生物的海洋18SrDNA序列揭示了未知的真核生物多样性
S Y Moon-van der Staay1, R De Wachter, D Vaulot
1Station Biologique, UPR 9042 Centre National de la Recherche Scientifique et Université Pierre et Marie Curie, Roscoff, France.
Nature
|February 24, 2001
概括
海洋picoeukaryotes,微小但重要的海洋细胞,显示高多样性. 这项研究使用了18S rRNA基因测序,揭示了太平洋中许多以前未知的真核生物浮游生物 (picoeukaryotes).
科学领域:
- 海洋生物学 海洋生物学
- 微生物生态学 微生物生态学
- 分子生物学分子生物学
背景情况:
- 在海洋初级生产和生物质中占主导地位的是小浮游生物.
- 与原核生物相比,皮可浮游生物的真核组成部分仍然不太了解.
- 传统的方法很难识别小浮游生物,因为它们的体积很小,缺乏明显的特征.
研究的目的:
- 为了研究赤道太平洋的picoeukaryotes的多样性.
- 应用分子技术,以更全面地分析小浮游生物的组成.
- 在海洋picoeukaryote组合中识别新的血统.
主要方法:
- 对35个全小子单元 (18S) 核糖体RNA基因序列的分析.
- 在赤道太平洋75米深处采集皮科浮游生物组合的样本.
- 使用分子方法对 Prokaryotes 成功,但对 Eukaryotes 不太常见.
主要成果:
- 发现了大量多样化的picoeukaryotes.
- 大多数被测序的18S rRNA基因都代表了以前未知的种群.
- 识别的序列属于主要的海洋类,包括普拉西诺菲特,哈普托菲特,恐龙鞭毛动物,斯特拉门诺皮尔,小阿诺鞭毛动物和鱼.
- 确定了一种与恐龙鞭毛动物相关的新血统.
结论:
- 分子分析揭示了海洋picoeukaryotes内显著的未开发的多样性.
- 18S rRNA基因测序方法对于表征复杂的微生物群落是有效的.
- 需要进一步的研究才能充分理解这些多样化的picoeukaryotes的生态作用.
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