来自Synechococcus elongatus的光系统II的晶体结构,分辨率为3.8A
1Max-Volmer-Institut für Biophysikalische Chemie und Biochemie, Technische Universität Berlin, Germany.
Nature
|February 24, 2001
概括
研究人员确定了光系统II的X射线结构,揭示了其蛋白质子单元和共因子的空间组织. 这一突破为在光合作用过程中氧化水至关重要的集群提供了新的见解.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 光合作用研究研究 光合作用研究
背景情况:
- 由光系统I和II驱动的氧气光合作用是地球的主要能量转化过程.
- 光系统II (PSII) 对于氧化水,提供电子,质子和释放氧气至关重要.
- 以前的结构数据缺乏分辨率或水氧化活性.
研究的目的:
- 为了确定活跃光系统II的高分辨率X射线结构.
- 在PSII中阐明蛋白质子单元和辅助因子的空间组织.
- 提供有关参与水氧化的集群的详细信息.
主要方法:
- 完全活跃的光系统II晶体的X射线晶体学.
- 对完整的PSII电子显微镜数据的分析.
- PSII碎片的电子晶体学.
主要成果:
- 确定了活跃光系统II的X射线结构.
- 发现了蛋白质子单元和辅助因子的空间组织.
- 确定了团的确切位置,方向和细节.
结论:
- 该研究提供了活性光系统II的详细结构模型.
- 了解团的结构,可以了解水的氧化机制.
- 这项工作推动了光合作用研究和生物能源学领域的发展.
相关概念视频
Photosystem II
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
Photosystem I
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
X-ray Crystallography
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
The Photochemical Reaction Center
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
Photosystems
Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
Determination of Crystal Structures
In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...


