纤维素信使RNA的大小及其聚亚丁烯酸含量
Cell
|March 1, 1975
概括
研究人员使用电子显微镜和电泳测量了纤维蛋白传递 RNA (mRNA) 的分子量. 他们发现,poly(A) 序列位于mRNA分子的3'端.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 纤维蛋白是丝中一个关键的结构蛋白.
- 了解信使RNA (mRNA) 的特征对于蛋白质合成研究至关重要.
研究的目的:
- 为了准确确定纤维素mRNA的分子量.
- 为了调查纤维蛋白mRNA中的多元A序列的存在和位置.
主要方法:
- 电子显微镜用于轮长度测量.
- 在变质条件下进行电泳.
- 奥利戈 (dT) -纤维素结合测定.
- 混合化和电子显微镜用于多种 (A) 定位.
主要成果:
- 纤维素mRNA分子重量确定为5.6-6.0 x 10^6达尔顿.
- 平均分子重量表明编码为414,000达尔顿的蛋白质.
- 43-81%的mRNA分子含有多元A序列.
- 多A序列包括~0.6%的分子 (~100个核酸).
- 在mRNA的3'端定位的多A) 序列.
结论:
- 纤维蛋白mRNA是一个大,同质的分子,当准备精心.
- 经证实,多A尾部位于纤维蛋白mRNA的3'端.
- 这一发现与聚A在mRNA稳定性和翻译中的作用相一致.
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