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相关概念视频

Establishment and Characterization of Patient-derived Gastric Organoids from Biopsies of Benign Gastric Body and Antral Epithelium08:45

Establishment and Characterization of Patient-derived Gastric Organoids from Biopsies of Benign Gastric Body and Antral Epithelium

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Gastric patient-derived organoids find increasing use in research, yet formal protocols for generating human gastric organoids from single-cell digests with standardized seeding density are lacking. This protocol presents a detailed method for reliably creating gastric organoids from biopsy tissue obtained during upper...
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Laparoscopy-endoscopy Cooperative Surgery for the Treatment of Gastric Gastrointestinal Stromal Tumors

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Laparoscopy-endoscopy cooperative surgery is appropriate for the treatment of gastric gastrointestinal stromal tumors less than 5 cm in size. It can achieve the respective advantages of endoscopic and laparoscopic surgeries while avoiding their...
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Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization08:16

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

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Multiplex in situ hybridization (ISH) was employed to simultaneously visualize the transcripts for two G protein-coupled receptors and one transcription factor in the entire vagal ganglionic complex of the adult mouse. This protocol could be used to generate accurate maps of the transcriptional profiles of vagal afferent...
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One-anastomosis Gastric Bypass (OAGB) in Rats08:16

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This protocol is for the performance of one-anastomosis gastric bypass (OAGB) on rat. The operator performs a long and tubular-stapled gastric pouch followed by hand-sewn anastomosis. For this model, the operator reproduces the same ratio between biliopancreatic and common limb in humans; therefore, the biliopancreatic limb measures 35 cm.
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Determination of gastric emptying with a non-invasive [13C]-octanoic acid breath test for tracking gastroparesis in female NOD LtJ...
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Numerous studies using gastric bypass rat models have been recently conducted to uncover the underlying physiological mechanisms of Roux-en-Y gastric bypass operations. This article aims to demonstrate and discuss the technical and experimental details of our published gastric bypass rat model to understand advantages and limitations of this experimental...
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相关实验视频

Updated: Feb 1, 2026

One-anastomosis Gastric Bypass OAGB in Rats
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一个基因组视角的膜区组织组织.

J B Bock1, H T Matern, A A Peden

  • 1Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, California 94305-5426, USA.

Nature
|March 10, 2001
PubMed
概括

研究人员研究了参与真核细胞膜组织的蛋白质家族. 拉布家族从酵母显著扩展到人类,SNARE蛋白质在人类中分解成四个不同的亚家族.

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 细胞生物学 细胞生物学

背景情况:

  • 细胞基因组测序为研究蛋白质家族进化提供了机会.
  • 了解膜区组织对于细胞生物学来说至关重要.

研究的目的:

  • 为了研究关键蛋白家族的进化扩张涉及到各种真核基因组的膜组织.
  • 识别和分类SNARE,外套复合体,Rab和Sec1蛋白家族的成员.

主要方法:

  • 对四种真核生物基因组 (酵母,,,人类) 的比较基因组学分析.
  • 蛋白质家族成员的识别和特征.
  • 遗传学分析以解决蛋白质子家族.

主要成果:

  • 拉布蛋白家族显示出从酵母到多细胞生物的持续扩张.
  • 所有研究的蛋白质家族,特别是SNAREs,Rabs和外套复杂子单元,在人类中显著扩大.
  • 该SNARE蛋白家族被分解成四个不同的子家族.

结论:

  • 蛋白质家族扩张,特别是Rabs和SNARE,是塑造真核细胞膜组织的关键进化机制.

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  • 人类细胞具有高度扩展的蛋白质谱,涉及到膜贩运和隔间组织.