人类染色体12的高分辨率地图
K T Montgomery1, E Lee, A Miller
1Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Nature
|March 10, 2001
概括
一个新的染色体12的序列标记站点地图,与全基因组指纹集成,提供完整的细菌克隆覆盖. 这种集成映射协议可以作为构建整个基因组物理图的模型.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 大型基因组的物理映射对于遗传研究和理解基因组组织至关重要.
- 之前对12号染色体的测绘工作是分散的,阻碍了全面的分析.
研究的目的:
- 将序列标记位点 (STS) 地图与12号染色体的全基因组指纹进行整合.
- 使用细菌克隆,创建一个准确和几乎完整的染色体12的物理地图.
- 建立一个全基因组物理映射的模型协议.
主要方法:
- 开发和应用序列标记网站 (STS) 内容映射.
- 将STS映射数据与全基因组指纹数据集成.
- 使用细菌人工染色体 (BAC) 克隆进行物理映射.
主要成果:
- 染色体12的STS内容图与全基因组指纹数据的成功整合.
- 实现了对12号染色体的细菌克隆的准确和几乎完整的覆盖.
- 证明了集成映射协议的稳定性.
结论:
- 综合映射协议提供了12号染色体的高度准确的物理地图.
- 这种方法可以作为一个可扩展的模型,用于构建整个基因组的物理地图.
- 促进未来的基因组研究和比较基因组学.
相关概念视频
01:17Karyotyping
Overview
02:40Chromosome Structure
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
01:17Karyotyping
Overview
01:51Lampbrush Chromosomes
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...
02:40Chromosome Structure
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
01:51Lampbrush Chromosomes
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops resemble the...