相关实验视频
Updated: May 12, 2026
10:28
Body Composition and Metabolic Caging Analysis in High Fat Fed Mice
Published on: May 24, 2018
缺乏M3肌性乙胆受体的小鼠是低和瘦的
M Yamada1, T Miyakawa, A Duttaroy
1Laboratory of Bioorganic Chemistry, RIKEN Brain Science Institute, Wako-shi, Saitama 351-0198, Japan.
Nature
|March 10, 2001
概括
缺乏M3肌性受体 (M3R) 的小鼠显示食物摄入量和体重减少. 这表明一种涉及M3受体的胆性通路促进了独立于MCH系统的食.
科学领域:
- 神经科学是一个神经科学.
- 生理学 生理学 生理学
- 药理学 药理学是指药理学的学科.
背景情况:
- 肌肉酸乙胆受体 (M1-M5) 调节重要的生理功能.
- 鉴定介导乙胆作用的特定受体亚型对于治疗至关重要,但由于缺乏选择性连接体,具有挑战性.
研究的目的:
- 研究M3肌糖受体 (M3R) 在调节食物摄入量和体重方面的作用.
- 阐明M3R在中央养通路中的潜在参与.
主要方法:
- 生成和研究的小鼠缺乏M3肌糖受体 (M3R-/-小鼠).
- 评估食物摄入量,体重,血清瘦素和胰岛素水平.
- 测量下丘脑黑色素缩激素 (MCH) 的mRNA水平.
- 进行了与agouti相关的类同类物和MCH的脑内静脉注射.
主要成果:
- M3R-/-小鼠的食物摄入量显著减少,体重减轻,以及较低的外围脂肪沉积.
- 在M3R-/-小鼠中,血清瘦素和胰岛素水平显著降低.
- 在M3R-/-小鼠中,心下MCH mRNA水平矛盾地降低了.
- 与Agouti相关的类似物在M3R-/-小鼠中失去了氧化活性,而MCH保留了刺激食欲的效果.
结论:
- 肌肉蛋白受体M3在促进食物摄入方面发挥着重要作用.
- 一个涉及M3R的胆固醇通路似乎调节了叶丁/黑色皮质素系统的下游和MCH系统的上游的养.
- 这些发现凸显了M3R作为食欲调节的潜在治疗点.
相关概念视频
02:41Lethal Alleles
Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
01:25Cholinergic Receptors: Muscarinic
The pharmacological actions of acetylcholine are elicited via its binding to two families of cholinergic receptors or cholinoceptors, namely, muscarinic and nicotinic receptors. Muscarinic receptors are G protein-coupled receptors and have five subtypes, M1–M5. All mAChR subtypes are activated by acetylcholine and blocked by the antagonist, atropine.
The subtypes M1, M3, and M5 couple with the Gq subunit and activate the phospholipase C (PLC) activity, mobilizing intracellular Ca2+. Activation...
The subtypes M1, M3, and M5 couple with the Gq subunit and activate the phospholipase C (PLC) activity, mobilizing intracellular Ca2+. Activation...