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波罗样类激酶1酸化环素B1并在预相过程中将其向细胞核
F Toyoshima-Morimoto1, E Taniguchi, N Shinya
1Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Nature
|March 10, 2001
概括
波罗样类激酶1 (Plk1) 酸化环素B1,这是细胞分裂的关键蛋白质. 这种酸化对于循环蛋白B1进入细胞核至关重要,调节脊椎动物细胞M相事件.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- Cdc2-cyclin B1 (MPF) 的核进入对于M相事件至关重要.
- 负责环林B1酸化和核转位的基因酶是未知的.
研究的目的:
- 确定负责在147 (S147) 血清中酸化环林B1的激酶.
- 研究这种激酶在预相过程中在环林B1核进口中的作用.
主要方法:
- 从Xenopus M相提取物中净化蛋白激酶.
- 鉴定酶为Plx1,这是Polo样酶 (Plk) -1的同类物.
- 在体外激酶测定,抗体减少,以及在HeLa细胞中的突变性环林B1分析.
主要成果:
- 在B1核出口信号中,纯化酶酸化S147.
- 在细胞周期进展过程中,Plk1活性与环林B1酸化相关.
- 突变型环林B1 (S133A/S147A) 无法进入核中,而野生型环林B1则在核中积累.
- 活性Plk1刺激环素B1的核进口.
结论:
- Plk1在S147上化环素B1,促进其核转移.
- Plk1在预相期间将MPF向细胞核中起作用.
- 这种机制在脊椎动物细胞中得到保存.
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