酵母的形状多样性决定了它的播种特异性
1Department of Cellular and Molecular Pharmacology, University of California-San Francisco, 94143-0450, USA.
Nature
|March 10, 2001
概括
子菌株,就像酵母[PSI+]子一样,源于蛋白质内部的自我传播的结构变化. 这些结构变异影响着子的行为和跨物种传播障碍.
科学领域:
- 分子生物学分子生物学
- 子生物学 子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 子表现出菌株多样性,甚至在相同的主体中产生不同的表型.
- 子菌株多样性和物种间传播障碍的分子基础尚未完全理解.
研究的目的:
- 调查构造差异在子菌株多样性中的作用.
- 检查这些差异如何影响子传输.
主要方法:
- 从Saccharomyces cerevisiae和Candida albicans Sup35.35的子域中创建了一个嵌合蛋白.
- 在体内通过短暂的过度表达诱导子菌株.
- 使用种子测试在体外传播出不同的粉样蛋白构造.
主要成果:
- 嵌合体Sup35在体内形成了替代的子菌株,取决于启动Sup35的物种.
- 在实验室中,当与不同的Sup35纤维进行种植时,喜梅拉会繁殖出不同的粉样蛋白形状.
- 粉样纤维构造确定了播种特异性,显示了物种依赖的传播.
结论:
- 可遗传的子菌株是由子蛋白内自我传播的构造差异造成的.
- 形状变化,以及主要蛋白质结构,决定了子的物种传播障碍.
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