EGF受体和Notch信号传递作用于Eyeless/Pax6的上游,以控制眼睛的特异性
1Department of Cell Biology, Emory University School of Medicine, 1648 Pierce Drive, Atlanta, GA 30322, USA.
Cell
|March 21, 2001
概括
对于Drosophila眼睛发育的主控制基因在早期没有共同表达. 相反,EGF受体和Notch信号通路稍后在第二个幼虫阶段启动眼睛的特异化.
科学领域:
- 发育生物学是发展生物学.
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 德洛索菲拉复合眼睛通过七个核因子的作用而发展,包括无眼/Pax6,称为主控制蛋白.
- 已知这些因素在异位表达时会诱导眼睛的发育,而在突变时会导致眼睛损失.
- 以前的理解表明,这些基因在胚胎假定眼中被共同表达.
研究的目的:
- 为了研究眼睛特异性基因在Drosophila的精确时间和细胞共同表达.
- 为了确定参与眼睛发育的主控制基因的上游调节者.
- 为了确定什么时候眼睛的规范真正发生在Drosophila发育过程中.
主要方法:
- 基因表达模式的遗传分析.
- 调查EGF受体和Notch信号通路的作用.
- 关键发育基因的功能丧失和宫外表达研究.
主要成果:
- 几个关键的眼睛特异性基因在相同的胚胎细胞或假定眼睛中没有共同表达.
- 皮表皮生长因子 (EGF) 受体和Notch信号通路在眼睛特异性基因的上游运行.
- 已经证明,眼睛的特异性发生在第二个幼虫阶段,而不是胚胎发育期间.
结论:
- 在Drosophila中,眼睛的特异化是一个比以前认为的晚期的过程.
- EGF受体和Notch信号通路在启动眼睛发育方面发挥着关键的同源作用.
- 眼睛特异性基因的空间和时间表达模式比最初建议的主控制模型更复杂.
相关概念视频
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
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03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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