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Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...

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相关实验视频

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Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
12:36

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 15, 2016

在Arabidopsis thaliana中通过环素40调节植物阶段的变化.

T Z Berardini1, K Bollman, H Sun

  • 1Plant Science Institute, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104-6018, USA. spoethig@sas.upenn.edu

Science (New York, N.Y.)
|March 27, 2001
PubMed
概括

植物发芽的成熟包括植物和生殖阶段. 编码环素40 (CyP40) 的SQUINT (SQN) 基因对于植物成熟至关重要,但对于Arabidopsis的繁殖能力至关重要.

科学领域:

  • 植物生物学 植物生物学
  • 发育生物学是发展生物学.
  • 分子遗传学 分子遗传学

背景情况:

  • 植物发芽的发展涉及从幼年到成年植物生长的过渡,以及从生殖无能到能力的过渡.
  • SQUINT (SQN) 基因在这些发育过渡中的作用尚未完全理解.

研究的目的:

  • 调查SQUINT (SQN) 在植物芽成熟中的功能.
  • 确定SQN是否参与植物和/或生殖发育.

主要方法:

  • 利用Arabidopsis中SQUINT (SQN) 基因中的功能丧失突变.
  • 分析这些突变对发芽形态,叶子发育,开花时间和生殖能力的影响.

主要成果:

  • 在SQN中功能丧失突变减少了幼叶的数量.
  • SQN突变对花朵形态有微妙的影响.
  • 没有观察到对开花时间或生殖能力的显著影响.
  • SQN编码的是环素40 (CyP40) 的阿拉比多普西斯同类物,它是Hsp90护航者复合物的组成部分.

结论:

  • 在阿拉比多普西斯中,由SQN编码的CyP40蛋白质特别需要用于植物芽的成熟.

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  • 对于植物芽的繁殖成熟,CyP40不是必不可少的.