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In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

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Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent...
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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression08:54

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This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator...
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A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer11:53

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This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this...
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Generation of Genetically Modified Mice through the Microinjection of Oocytes10:19

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The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping...
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DNA Vector-based RNA Interference to Study Gene Function in Cancer13:10

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RNA interference (RNAi) possesses many advantages over gene knockout and has been broadly used as a tool in gene functional studies. The invention of DNA vector-based RNAi technology has made long term and inducible gene knockdown possible, and also increased the feasibility of gene silencing in...
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We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C.
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相关实验视频

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Utilizing Percutaneous Ventricular Assist Devices in Acute Myocardial Infarction Complicated by Cardiogenic Shock
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验证一种临床决策辅助器,用于停止医院内心脏骤停复苏.

C van Walraven1, A J Forster, D C Parish

  • 1Department of Medicine, University of Ottawa, Ontario, Canada. carlv@lri.ca

JAMA
|April 13, 2001
PubMed
概括
此摘要是机器生成的。

一个新的临床决策辅助器准确地识别出可能在医院内存活心脏复苏的患者. 这种工具有助于医生确定何时持续的复苏工作可能对患者没有好处,从而改善了资源配置.

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科学领域:

  • 紧急医疗 紧急医疗
  • 心脏病学 心脏病学
  • 临床决策支持 临床决策支持

背景情况:

  • 在医院内心脏复苏的生存率仍然很低.
  • 之前的一项研究开发了一种临床决策辅助器,用于预测出院后的存活率.
  • 这项研究旨在验证此前获得的决策援助.

研究的目的:

  • 验证一项先前开发的临床决策辅助工具,用于预测在医院内心脏复苏后在医院出院后的存活率.
  • 评估决策辅助的准确性,以确定不太可能从持续复苏中受益的患者.

主要方法:

  • 从1987年至1996年,对一大批住院心脏复苏尝试 (n=2181) 的注册表进行分析.
  • 决策辅助器预测了基于目击停顿,初始心律 (心室高心率/心肌) 或在10分钟内恢复的脉的生存率.
  • 预测与实际的医院出院结果进行了比较.

主要成果:

  • 对于99.1%的幸存者来说,决策辅助正确预测了存活到出院的时间 (敏感度).
  • 对于预测没有生存机会的患者 (n=269),只有1.1%的患者得到了出院,没有人独立出院.
  • 援助的负预测值为98.9%.

结论:

  • 经过验证的临床决策辅助器准确地识别出不太可能在医院内存活心脏复苏的患者.
  • 这个工具可以帮助医生做出明智的决定,继续进行复苏工作.
  • 该援助支持更好的资源配置,通过识别具有极低有利结果可能性的患者.