碳水化合物位点在阿尔法病毒糖蛋白上的位置表明,E1形成了一个二元体结构的支架
Sergei V Pletnev1, Wei Zhang1, Suchetana Mukhopadhyay1
1Department of Biological Sciences Purdue University West Lafayette, Indiana 47907.
Cell
|April 13, 2001
概括
辛迪斯病毒有80个表面尖峰,由糖蛋白E1和E2组成. 研究人员绘制了糖化位点的地图,揭示了E2形成尖峰,E1形成了二元体支架,这表明与黄病毒有类似的融合机制.
科学领域:
- 病毒学 病毒学
- 结构生物学 结构生物学
- 分子生物学分子生物学
背景情况:
- 辛德比斯病毒具有80个表面尖峰,排列在一个二面体格子中.
- 每个尖峰都是E1和E2糖蛋白的三元体,每个尖峰都有两个糖化位点.
研究的目的:
- 为了确定Sindbis病毒糖蛋白E1和E2.2上糖化位的确切位置.
- 阐明Sindbis病毒表面糖蛋白的结构组织及其在病毒融合中的作用.
主要方法:
- 用特定位点的突变发生法来去除糖基化识别动机.
- 使用冷电子显微镜可视化了糖蛋白的结构布局.
主要成果:
- 精确地绘制了四个糖化位点的位置 (两个在E1,两个在E2).
- 鉴定出E2糖蛋白是形成突出的尖的.
- 发现E1糖蛋白是长而窄的,平坦地躺着形成一个icosahedral支架,类似于flavivirus E糖蛋白.
结论:
- 在Sindbis病毒中,E1和E2糖蛋白的结构安排是不同的,E2形成尖峰,E1形成支架.
- 鉴定到的E1的分子间接触表明,它的融合机制类似于病毒的融合机制.
- 这项研究提供了关于病毒融合过程的见解,以及alphavirus-flavivirus相似性的结构基础.
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The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
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