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相关概念视频

DNA Helicases00:55

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

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相关实验视频

Updated: May 12, 2026

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
08:53

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Published on: February 18, 2011

BACH1是一种新型的类似酶的蛋白质,直接与BRCA1相互作用,并有助于其DNA修复功能.

S B Cantor1, D W Bell, S Ganesan

  • 1The Dana-Farber Cancer Institute and the Harvard, Medical School, Boston, MA 02115, USA.

Cell
|April 13, 2001
PubMed
概括

研究人员发现BACH1,一个DEAH螺旋酶,与BRCA1.1相互作用. 这种相互作用对DNA修复至关重要,BACH1突变与早期发病的乳腺癌有关,这表明它也可能是致癌基因.

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Visualization of DNA Repair Proteins Interaction by Immunofluorescence
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Visualization of DNA Repair Proteins Interaction by Immunofluorescence

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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

相关实验视频

Last Updated: May 12, 2026

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
08:53

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Published on: February 18, 2011

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 生物化学 生物化学

背景情况:

  • BRCA1是一种关键的瘤抑制基因,参与DNA修复.
  • DEAH螺旋酶家族在各种细胞过程中发挥作用,包括DNA代谢.

研究的目的:

  • 为了识别新的BRCA1相互作用蛋白.
  • 研究BACH1在DNA修复中的功能作用及其在癌症中的潜在参与.

主要方法:

  • 同免疫沉检测在体内BRCA1和BACH1.1之间的相互作用.
  • 局部定向的突变发生,以产生一种催化无活性的BACH1衍生物.
  • 彗星测试以评估双链断裂修复效率.
  • 胚胎DNA测序用于识别乳腺癌患者的BACH1突变.

主要成果:

  • BACH1,一个DEAH酶,被确定为一种新的BRCA1相互作用蛋白.
  • BACH1直接与BRCA1.1的BRCT重复结合.
  • 一种触媒不活跃的BACH1突变物以BRCA1依赖的方式损害了DNA双链断裂修复.
  • 在两个早期发病的乳腺癌患者中发现了BACH1螺旋酶域中的生殖系突变.

结论:

  • 形成BACH1/BRCA1复合体对于BRCA1在DNA修复中的关键功能至关重要.
  • BACH1可能起到瘤抑制作用,而BACH1的生殖系突变可能会导致乳腺癌的发展,类似于BRCA1.