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Analyzing Murine Schwann Cell Development Along Growing Axons
Published on: November 21, 2012
神经Y作为神经扩散因子起作用
D E Hansel1, B A Eipper, G V Ronnett
1Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Nature
|April 20, 2001
概括
神经Y (NPY) 促进嗅觉系统中新神经元的增殖. 这一发现揭示了NPY在成人神经发生和支架细胞功能中的新作用.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 身体生理学 身体生理学
背景情况:
- 神经Y (NPY) 已知在哺乳动物生理学中的不同作用.
- 嗅觉上皮质中的支架细胞传统上被认为是结构支持细胞.
研究的目的:
- 研究神经Y (NPY) 在产后神经前体细胞的增殖中的作用.
- 为了确定参与NPY介导神经发生的细胞源和信号通路.
主要方法:
- 利用小鼠的向基因删除模型来研究NPY缺陷的表型.
- 研究了细胞外信号调节激酶 (ERK) 1/2和蛋白激酶C (PKC) 信号通路的激活.
- 使用显微镜和细胞计数技术检查了嗅觉上皮的细胞增殖.
主要成果:
- 缺乏NPY的小鼠,嗅觉神经元前体细胞分裂率降低了50%.
- 与对照组相比,成年NPY缺陷小鼠的嗅觉神经元显著减少.
- 在神经元前体细胞中,NPY迅速和暂时激活ERK1/2,通过NPY Y1受体和上游PKC信号传递进行中介.
结论:
- 神经Y (NPY) 在促进嗅觉上皮质中产后神经元前体细胞的增殖方面发挥着至关重要的作用.
- 支架细胞合成NPY,表明结构支持之外的新功能.
- 涉及Y1受体,PKC和ERK1/2的NPY信号调节成人神经发生.
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