在Drosophila昼夜振荡器中,加密染色体的新作用
B Krishnan1, J D Levine, M K Lynch
1Department of Biology and Biochemistry and Biological Clocks Program, University of Houston, Houston, Texas 77204, USA.
Nature
|May 18, 2001
概括
加密染色体 (CRY) 对于昼夜时钟的功能至关重要. 在Drosophila中,CRY在外周振荡器,特别是天线中具有光受体独立的作用,揭示了中央和外周时钟机制之间的差异.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 昆虫生理学 昆虫生理学
背景情况:
- 加密染色体 (CRY) 是flavin/pterin蛋白质,对Drosophila和小鼠的昼夜时钟机制至关重要.
- 在Drosophila中,CRY作为光受体,用于输入到大脑和外围组织中的昼夜振荡器中的光.
- 一个Drosophila哭泣突变体 (cryb) 破坏了外周振荡器功能,导致嗅觉反应的失律.
研究的目的:
- 为了研究加密色素 (CRY) 在Drosophila天线中的外周昼夜振荡器功能中的作用.
- 为了确定CRY在外围的作用是否依赖光感受器或独立.
- 探索Drosophila中中央和外围昼夜振荡器机制之间的差异.
主要方法:
- 训练Drosophila进行光暗周期和温度周期.
- 评估天中的昼夜嗅觉反应.
- 分析了野生类型和巨突变中的外围振荡器的功能.
主要成果:
- 加密色 (CRY) 促成振荡器功能和生理输出节奏在Drosophila天线.
- 即使光学输入被消除,通过将引入温度周期,这种作用仍然存在.
- 克里布突变体表现出被破坏的外周振荡器功能,表明CRY的重要性超出了光接收.
结论:
- 加密色 (CRY) 在Drosophila天线的外周昼夜振荡器中扮演着光受体独立的角色.
- 这一发现表明Drosophila中中央和外围昼夜振荡器的分子机制之间的根本差异.
- 在外围组织中CRY的功能突出显示了它对昼夜调节的多方面的贡献.
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