埃米1是一种线粒调节剂,它与Cdc20相互作用,并抑制促进复杂性亚纳相的亚纳相
J D Reimann1, E Freed, J Y Hsu
1Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA.
Cell
|June 8, 2001
概括
我们发现了Emi1,一种早期的线粒酶抑制剂,可以防止过早的亚纳相促进复合体/循环体 (APC) 激活. 这种调节对于在线粒分裂过程中及时进行细胞循环进展至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 分解是一种由复杂的分子机械调节的关键细胞分裂过程.
- 亚纳酶促进复合体/循环体 (APC) 是一个关键的E3泛基因酶,控制细胞循环的进展.
- 线粒细胞进展的失调会导致严重的细胞缺陷和疾病.
研究的目的:
- 为了识别和表征早期线粒分裂的新型调节者.
- 阐明 Emi1 控制 APC 活动的机制.
- 了解Emi1在细胞周期进展中的作用.
主要方法:
- 奇诺普斯蛋提取物被用来研究细胞循环调节.
- 免疫减弱和表达非可降解的EMI1被使用.
- 进行了共免疫沉试验,以研究蛋白质相互作用.
主要成果:
- 艾米1是一种F盒蛋白,通过其结合域抑制APC活性.
- 艾米1在线粒分裂之前积累,在线粒分裂期间被降解,独立于APC.
- 艾米1的枯竭延迟了线粒体的进入,而不可降解的艾米1则通过稳定APC基质而导致线粒体的阻断.
- 艾米1与Cdc20,一种APC激活剂相互作用,而Cdc20可以覆盖Emi1诱导的循环蛋白B破坏的抑制.
结论:
- 艾米1通过防止过早的APC激活,起到早期线索性抑制剂的作用.
- 艾米1是线粒细胞进展时间的关键调节者.
- 埃米1功能提供了关于循环蛋白B/Cdc2激活和循环蛋白B在线粒分裂中的破坏之间的延迟的见解.
相关概念视频
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:17Separation of Sister Chromatids
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...