一种自由扩散的Sonic hedgehog形式介导长距离的信号传输
1Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, Ohio 45267, USA.
Nature
|June 8, 2001
概括
研究人员发现了一种自由扩散的Sonic hedgehog (Shh) 形式,称为s-ShhNp,对于发育中的远程信号至关重要. 它的可用性是由路径对抗剂调节的,它在小的四肢中形成了梯度.
科学领域:
- 发育生物学 发展生物学
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 声波刺 (Shh) 是一种对胚胎发育至关重要的分泌蛋白质,通过短距离和远距离信号传输来调解模式.
- 远程Shh信号传输模型包括简单的扩散,信号中继和细胞系介导的传输.
- 以前的证据主要支持使用可溶性ShhN的扩散,对本地,自由扩散的Shh.有有限的数据.
研究的目的:
- 调查Sonic hedgehog (Shh) 的一种自由扩散的本地形式的存在和特征.
- 确定控制这种可扩散Shh形式的可用性的监管机制.
- 评估胚胎模式中自由扩散的Shh形式的生理相关性.
主要方法:
- 一种新型的生物化学特征,自由扩散的Shh形式 (s-ShhNp).
- 评估s-ShhNp的生物效能和多重体状态.
- 通过Shh通路对抗剂Patched (Ptc) 和刺相互作用蛋白 (Hip) 调节的分析.
- 在子四肢发育中的s-ShhNp梯度的检测.
主要成果:
- 提出的证据表明一种胆固醇修饰的,多重的,生物强大的自由扩散形式的Shh (s-ShhNp).
- s-ShhNp的可用性被证明是通过对抗剂Ptc和Hip进行调节的.
- 在小肢的前后轴上观察到s-ShhNp的梯度.
结论:
- 一种新的,自由扩散的,生物活跃的Sonic hedgehog (s-ShhNp) 已被确定.
- s-ShhNp的可用性受到Shh通路中的关键对手的积极调节.
- s-ShhNp梯度的存在证实了它在胚胎模式,特别是肢体发育中的生理作用.
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