通过代谢工程来对一个有义务的光自otrophic 生物体进行热带转化
L A Zaslavskaia1, J C Lippmeier, C Shih
1Martek Biosciences Corp., 6480 Dobbin Road, Columbia, MD 21045, USA.
概括
工程微藻 Phaeodactylum tricornutum 现在可以在没有光的情况下使用葡萄糖生长. 引入一种葡萄糖转运基因使这种代谢转变成为可能,为藻类发酵技术铺平了道路.
科学领域:
- * 生物技术和合成生物学
- * 微藻生理学和新陈代谢
背景情况:
- *大多数微藻类是有义务的光自营养,需要光来产生能量.
- *依赖光的生长限制了微藻的大规模种植和商业应用.
研究的目的:
- * 为了研究微藻在葡萄糖上的异质性生长的基因改造.
- *通过单基因引入来证明改变基本代谢途径的可行性.
主要方法:
- * 微藻 (Phaeodactylum tricornutum) 的基因工程.
- * 引入一种编码葡萄糖载体 (glut1或 hup1) 的基因.
- * 在没有光线的情况下,在外源葡萄糖上培育工程菌株.
主要成果:
- *成功设计了Phaeodactylum tricornutum以利用外源葡萄糖进行生长.
- * 在完全黑暗中表现出强大的生长,独立于光合作用.
- * 确认了引入的葡萄糖载体的功能表达.
结论:
- * 单个基因的引入可以从根本上改变微藻代谢,使异质.
- * 这一突破促进了光独立藻类培养的发展.
- * 在商业规模的藻类发酵技术方面取得了重大进展.
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