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Updated: Jun 21, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 18, 2010

评估蛋白质-蛋白质相互作用和酶-基质相互作用之间的平衡,在聚基酸合成酶模块之间的中间体的道化中

N Wu1, S Y Tsuji, D E Cane

  • 1Departments of Chemistry, Chemical Engineering, and Biochemistry, Stanford University, Stanford California 94305, USA.

Journal of the American Chemical Society
|July 6, 2001
PubMed
概括

在聚基化合成酶 (PKS) 中的基底道化显著提高了模块效率,特别是对于较差的基底. 这种机制使PKS模块能够处理更广泛的基质,释放组合生物合成的潜力.

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