来自RNA聚合酶β'亚单元的卷曲卷轴在全质上诱导通过sigma选择性的非模板链结合70)
B A Young1, L C Anthony, T M Gruber
1Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA.
Cell
|July 6, 2001
概括
来自RNA聚合酶β'亚单元的一种小使得sigma(70) 能够识别促进体DNA. 这种相互作用是转录启动的关键,模仿核心酶的效率.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 转录启动需要RNA聚合酶来识别和解促进DNA.
- 对于这个过程来说,选择性绑定到主办方的 -10 区域非模板链是非常重要的.
- 西格玛 (sigma) 70子单位在促进者识别中发挥作用.
研究的目的:
- 为了研究RNA聚合酶β'亚单元的特定区域在促进体识别中的作用.
- 要确定一个片段能否诱导sigma(70) 进行选择性非模板链结合.
- 阐明由β'子单元介导的sigma中性转换的机制.
主要方法:
- 研究了来自β'子单元 (aa 262-309) 的48个氨基酸卷曲-卷曲的功能.
- 评估了该诱导sigma(70) 结合促进子的非模板链的能力.
- 使用sigma的结构信息来理解相互作用机制.
主要成果:
- 贝塔'卷轴-卷轴 (aa 262-309) 诱导了sigma(70) 以接近核心RNA聚合酶效率与非模板链结合.
- 有证据表明,β'卷轴-卷轴和sigma ((70) 区域2.2.之间存在相互作用.
- 这种相互作用促进了sigma中的全过渡,使选择性非模板链识别成为可能.
- 非模板识别仅使用β'和Sigma的结晶部分 (70),代表全酶的一小部分.
结论:
- 来自RNA聚合酶β'亚单元的最小可以为sigma提供高效的促销器非模板链识别 (70).
- 在这个功能中,β'卷-卷和sigma{70) 区域2.2之间的相互作用至关重要.
- 这一发现通过识别促进体结合的关键组件,简化了对转录启动的理解.
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