一个转录活性复合体的APP与Fe65和基因酸转移酶Tip60的转录 [纠正的转录性]
1The Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, TX 75390-9111 USA.
概括
氨基酸β前体蛋白 (APP) 的细胞质尾部与Fe65和Tip60结合,激活基因转录. 这表明 APP 是 APP.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 粉样β前体蛋白 (APP) 被玛分泌酶分裂,释放出细胞内片段.
- 这种释放的APP细胞质尾部片段的生理功能目前尚不清楚.
- 阿尔茨海默氏症的特点是粉样β-的积累,来自APP.
研究的目的:
- 为了研究胺β前体蛋白 (APP) 的细胞质尾部的功能.
- 为了确定APP细胞质尾巴是否与其他细胞蛋白相互作用.
- 探索APP裂变产品在基因调节中的作用.
主要方法:
- 研究了APP细胞质尾巴与核蛋白的相互作用.
- 使用共免疫沉试验检测蛋白质复合体.
- 通过使用与Gal4-和LexA-DNA结合域的记者基因测定来评估转录活性.
主要成果:
- 证明了APP的细胞质尾巴与Fe65和Tip60.0形成了一个多重复合体.
- 表明这种APP-Fe65-Tip60复合物显著刺激转录.
- 表明 APP 的马分泌酶裂变释放了尾部片段,可能调节基因表达.
结论:
- 当被玛分泌酶释放时,APP的细胞质尾部可以与Fe65和Tip60.0形成一个功能复合体.
- 这种复合物作为一种强大的转录激活剂,表明APP细胞内片段在基因表达中的作用.
- 这些发现为APP及其加工产品的非amyloidogenic功能提供了新的见解.
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